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Engineering an Anti-Transferrin Receptor ScFv for pH-Sensitive Binding Leads to Increased Intracellular Accumulation

The equilibrium binding affinity of receptor-ligand or antibody-antigen pairs may be modulated by protonation of histidine side-chains, and such pH-dependent mechanisms play important roles in biological systems, affecting molecular uptake and trafficking. Here, we aimed to manipulate cellular trans...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Tillotson, Benjamin J., Goulatis, Loukas I., Parenti, Isabelle, Duxbury, Elizabeth, Shusta, Eric V.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4694649/
https://ncbi.nlm.nih.gov/pubmed/26713870
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0145820
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