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Ubiquitin‐Activated Interaction Traps (UBAITs) identify E3 ligase binding partners

We describe a new class of reagents for identifying substrates, adaptors, and regulators of HECT and RING E3s. UBAITs (Ubiquitin‐Activated Interaction Traps) are E3‐ubiquitin fusion proteins and, in an E1‐ and E2‐dependent manner, the C‐terminal ubiquitin moiety forms an amide linkage to proteins th...

詳細記述

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書誌詳細
出版年:EMBO Rep
主要な著者: O'Connor, Hazel F, Lyon, Nancy, Leung, Justin W, Agarwal, Poonam, Swaim, Caleb D, Miller, Kyle M, Huibregtse, Jon M
フォーマット: Artigo
言語:Inglês
出版事項: John Wiley and Sons Inc. 2015
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC4693525/
https://ncbi.nlm.nih.gov/pubmed/26508657
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15252/embr.201540620
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