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TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

Measuring levels of mRNAs in the process of translation in individual cells provides information on the proteins involved in cellular functions at a given point in time. The protocol dubbed Translating Ribosome Affinity Purification (TRAP) is able to capture this mRNA translation process in a cell-t...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Bertin, Benjamin, Renaud, Yoan, Aradhya, Rajaguru, Jagla, Krzysztof, Junion, Guillaume
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4692598/
https://ncbi.nlm.nih.gov/pubmed/26381166
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52985
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