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Assessing the Innate Sensing of HIV-1 Infected CD4(+) T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.
HIV-1 innate sensing requires direct contact of infected CD4(+) T cells with plasmacytoid dendritic cells (pDCs). In order to study this process, the protocols described here use freshly isolated human peripheral blood mononuclear cells (PBMCs) or plasmacytoid dendritic cells (pDCs) to sense infecti...
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| Publicado en: | J Vis Exp |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
MyJove Corporation
2015
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4692579/ https://ncbi.nlm.nih.gov/pubmed/26383704 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51207 |
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