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Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

This protocol describes a method to permit the tracking of cells through the cell cycle without requiring the cells to be synchronized. Achieving cell synchronization can be difficult for many cell systems. Standard practice is to block cell cycle progression at a specific stage and then release the...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Welschinger, Robert, Bendall, Linda J.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4692543/
https://ncbi.nlm.nih.gov/pubmed/26327278
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52840
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