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Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

This protocol describes a method to permit the tracking of cells through the cell cycle without requiring the cells to be synchronized. Achieving cell synchronization can be difficult for many cell systems. Standard practice is to block cell cycle progression at a specific stage and then release the...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:J Vis Exp
Hauptverfasser: Welschinger, Robert, Bendall, Linda J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: MyJove Corporation 2015
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4692543/
https://ncbi.nlm.nih.gov/pubmed/26327278
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52840
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