High-throughput sequencing of human plasma RNA by using thermostable group II intron reverse transcriptases

Next-generation RNA-sequencing (RNA-seq) has revolutionized transcriptome profiling, gene expression analysis, and RNA-based diagnostics. Here, we developed a new RNA-seq method that exploits thermostable group II intron reverse transcriptases (TGIRTs) and used it to profile human plasma RNAs. TGIRT...

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Bibliografiske detaljer
Udgivet i:RNA
Main Authors: Qin, Yidan, Yao, Jun, Wu, Douglas C., Nottingham, Ryan M., Mohr, Sabine, Hunicke-Smith, Scott, Lambowitz, Alan M.
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2016
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4691826/
https://ncbi.nlm.nih.gov/pubmed/26554030
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.054809.115
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