Directly interrogating single quantum dot labelled UvrA(2) molecules on DNA tightropes using an optically trapped nanoprobe

In this study we describe a new methodology to physically probe individual complexes formed between proteins and DNA. By combining nanoscale, high speed physical force measurement with sensitive fluorescence imaging we investigate the complex formed between the prokaryotic DNA repair protein UvrA(2)...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Simons, Michelle, Pollard, Mark R., Hughes, Craig D., Ward, Andrew D., Van Houten, Bennett, Towrie, Mike, Botchway, Stan W., Parker, Anthony W., Kad, Neil M.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4686980/
https://ncbi.nlm.nih.gov/pubmed/26691010
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep18486
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