A Plasmid-Transposon Hybrid Mutagenesis System Effective in a Broad Range of Enterobacteria

Random transposon mutagenesis is a powerful technique used to generate libraries of genetic insertions in many different bacterial strains. Here we develop a system facilitating random transposon mutagenesis in a range of different Gram-negative bacterial strains, including Pectobacterium atroseptic...

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Detalhes bibliográficos
Publicado no:Front Microbiol
Main Authors: Monson, Rita, Smith, Debra S., Matilla, Miguel A., Roberts, Kevin, Richardson, Elizabeth, Drew, Alison, Williamson, Neil, Ramsay, Josh, Welch, Martin, Salmond, George P. C.
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2015
Assuntos:
Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4686594/
https://ncbi.nlm.nih.gov/pubmed/26733980
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2015.01442
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