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New transposon delivery plasmids for insertional mutagenesis in Bacillus anthracis

Two new transposon delivery vector systems utilizing Mariner and mini-Tn10 transposons have been developed for in vivo insertional mutagenesis in Bacillus anthracis and other compatible Gram-positive species. The utility of both systems was directly demonstrated through the mutagenesis of a widely u...

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Detalles Bibliográficos
Main Authors: Wilson, Adam C., Perego, Marta, Hoch, James A.
Formato: Artigo
Idioma:Inglês
Publicado: 2007
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2175031/
https://ncbi.nlm.nih.gov/pubmed/17931726
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2007.09.006
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