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Identification of the nucleophile catalytic residue of GH51 α-l-arabinofuranosidase from Pleurotus ostreatus

In this study, the recombinant α-l-arabinofuranosidase from the fungus Pleurotus ostreatus (rPoAbf) was subjected to site-directed mutagenesis in order to identify the catalytic nucleophile residue. Based on bioinformatics and homology modelling analyses, E449 was revealed to be the potential nucleo...

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Bibliografski detalji
Izdano u:AMB Express
Glavni autori: Amore, Antonella, Iadonisi, Alfonso, Vincent, Florence, Faraco, Vincenza
Format: Artigo
Jezik:Inglês
Izdano: Springer Berlin Heidelberg 2015
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4686458/
https://ncbi.nlm.nih.gov/pubmed/26690659
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13568-015-0164-x
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