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Purification and characterisation of recombinant His-tagged RgpB gingipain from Porphymonas gingivalis

Gingipain proteases are important virulence factors from the periodontal pathogen Porphyromonas gingivalis and are the target of many in vitro studies. Due to their close biochemical properties, purification of individual gingipains is difficult and requires multiple chromatographic steps. In this s...

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Detalhes bibliográficos
Publicado no:Biol Chem
Main Authors: Veillard, Florian, Potempa, Barbara, Guo, Yonghua, Ksiazek, Miroslaw, Sztukowska, Maryta N., Houston, John A., Koneru, Lahari, Nguyen, Ky-Anh, Potempa, Jan
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4682895/
https://ncbi.nlm.nih.gov/pubmed/25720118
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1515/hsz-2014-0304
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