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Efficient preparation and properties of mRNAs containing a fluorescent cap analog: Anthraniloyl-m(7)GpppG

A method has been developed for synthesising fluorescently labeled capped mRNA. The method incorporates a single fluorescent molecule as part of the 5′-mRNA or oligonucleotide cap site. The fluorescent molecule, Ant-m(7)GTP is specifically incorporated into the cap site to yield Ant-m(7)GpppG-capped...

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Bibliografske podrobnosti
izdano v:Translation (Austin)
Main Authors: Gunawardana, Dilantha, Domashevskiy, Artem V, Gayler, Ken R, Goss, Dixie J
Format: Artigo
Jezik:Inglês
Izdano: Taylor & Francis 2015
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4682804/
https://ncbi.nlm.nih.gov/pubmed/26779415
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/21690731.2014.988538
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