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DNA-binding domain fusions enhance the targeting range and precision of Cas9

The CRISPR-Cas9 system is commonly employed in biomedical research; however, the precision of Cas9 is sub-optimal for gene therapy applications that involve editing a large population of cells. Variations on the standard Cas9 system have yielded improvements in the precision of targeted DNA cleavage...

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Detalhes bibliográficos
Publicado no:Nat Methods
Main Authors: Bolukbasi, Mehmet Fatih, Gupta, Ankit, Oikemus, Sarah, Derr, Alan G., Garber, Manuel, Brodsky, Michael H., Zhu, Lihua Julie, Wolfe, Scot A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4679368/
https://ncbi.nlm.nih.gov/pubmed/26480473
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3624
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