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Preparation of a mannose-6-phosphate glycan microarray through fluorescent derivatization, phosphorylation, and immobilization of natural high-mannose N-glycans and its application in ligand identification of P-type lectins
Glycan microarrays prepared by immobilization of amino-functionalized glycans on NHS-activated glass slides have been successfully used to study protein-glycan interactions. Fluorescently tagged glycans with an amino functional group can be prepared from natural glycans released from glycoproteins....
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| Published in: | Methods Mol Biol |
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| Main Authors: | , , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
2012
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4668285/ https://ncbi.nlm.nih.gov/pubmed/22057522 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61779-373-8_9 |
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