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In vitro and in vivo functional characterization of bovine vitamin K-dependent gamma-carboxylase expressed in Chinese hamster ovary cells.

Coagulation factor IX is a serine protease for which high-level expression of biologically active protein in heterologous cells is limited due to inefficient proteolytic removal of the propeptide as well as vitamin K-dependent carboxylation of multiple amino-terminal glutamic acid residues. We have...

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Publicado no:Proc Natl Acad Sci U S A
Principais autores: Rehemtulla, A, Roth, D A, Wasley, L C, Kuliopulos, A, Walsh, C T, Furie, B, Furie, B C, Kaufman, R J
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1993
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC46562/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8506307/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.10.4611
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