Direct uptake of Antagomirs and efficient knockdown of miRNA in primary B and T lymphocytes

Depending on their physiological expression level, microRNAs (miRNA) address different target genes, thus have different biological functions. To identify these, the physiological expression has to be blocked. Here, we describe the use of inhibitory cholesterol-modified oligonucleotides (Antagomirs)...

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Bibliographic Details
Published in:J Immunol Methods
Main Authors: Haftmann, Claudia, Riedel, René, Porstner, Martina, Wittmann, Jürgen, Chang, Hyun-Dong, Radbruch, Andreas, Mashreghi, Mir-Farzin
Format: Artigo
Language:Inglês
Published: Elsevier 2015
Subjects:
Technical Note
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4655414/
https://ncbi.nlm.nih.gov/pubmed/26187895
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2015.07.006
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