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Effects of transcription elongation rate and Xrn2 exonuclease activity on RNA polymerase II termination suggest widespread kinetic competition

The torpedo model of transcription termination asserts that the exonuclease Xrn2 attacks the 5′PO(4)-end exposed by nascent RNA cleavage and chases down the RNA polymerase. We tested this mechanism using a dominant-negative human Xrn2 mutant and found that it delayed termination genome-wide. Xrn2 nu...

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Detalhes bibliográficos
Publicado no:Mol Cell
Main Authors: Fong, Nova, Brannan, Kristopher, Erickson, Benjamin, Kim, Hyunmin, Cortazar, Michael, Sheridan, Ryan M., Nguyen, Tram, Karp, Shai, Bentley, David L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4654110/
https://ncbi.nlm.nih.gov/pubmed/26474067
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2015.09.026
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