Comparison of Different Buffers for Protein Extraction from Formalin-Fixed and Paraffin-Embedded Tissue Specimens

We determined the best extraction buffer for proteomic investigation using formalin-fixation and paraffin-embedded (FFPE) specimens. A Zwittergent 3–16 based buffer, sodium dodecyl sulfate (SDS)-containing buffer with/without polyethylene glycol 20000 (PEG20000), urea-containing buffer, and FFPE-FAS...

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Udgivet i:PLoS One
Main Authors: Shen, Kaini, Sun, Jian, Cao, Xinxin, Zhou, Daobin, Li, Jian
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2015
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4651363/
https://ncbi.nlm.nih.gov/pubmed/26580073
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0142650
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