Efficient DNA Extraction for HPV Genotyping in Formalin-Fixed, Paraffin-Embedded Tissues

DNA from archived FFPE can be used for papillomavirus genotyping, but potential problems include paraffin as a physical barrier, DNA cross-linking, and PCR inhibitors. To address these complications, we combined a commercially available DNA isolation kit (Qiagen DNeasy) with a heat treatment and eva...

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Bibliografiske detaljer
Main Authors: Steinau, Martin, Patel, Sonya S., Unger, Elizabeth R.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Investigative Pathology 2011
Fag:
Technical Advance
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3123789/
https://ncbi.nlm.nih.gov/pubmed/21704270
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmoldx.2011.03.007
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