An integrated approach for identifying and mapping human genes.

We have developed a method for generating expressed-sequence maps of human chromosomes. The method involves several steps that begin with libraries of highly representative short cDNAs prepared by using random oligomers as primers. The cDNA inserts are amplified by PCR with flanking vector primers....

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Detalhes bibliográficos
Main Authors: Das Gupta, R, Morrow, B, Marondel, I, Parimoo, S, Goei, V L, Gruen, J, Weissman, S, Skoultchi, A, Kucherlapati, R
Formato: Artigo
Idioma:Inglês
Publicado em: 1993
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC46511/
https://ncbi.nlm.nih.gov/pubmed/8506274
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