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Characterization and directed evolution of a methyl-binding domain protein for high-sensitivity DNA methylation analysis
Methyl-binding domain (MBD) family proteins specifically bind double-stranded, methylated DNA which makes them useful for DNA methylation analysis. We displayed three of the core members MBD1, MBD2 and MBD4 on the surface of Saccharomyces cerevisiae cells. Using the yeast display platform, we determ...
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| Publicado no: | Protein Eng Des Sel |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4646160/ https://ncbi.nlm.nih.gov/pubmed/26384511 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzv046 |
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