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High resolution 4D HPCH experiment for sequential assignment of (13)C-labeled RNAs via phosphodiester backbone

The three-dimensional structure determination of RNAs by NMR spectroscopy requires sequential resonance assignment, often hampered by assignment ambiguities and limited dispersion of (1)H and (13)C chemical shifts, especially of C4′/H4′. Here we present a novel through-bond 4D HPCH NMR experiment in...

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Dades bibliogràfiques
Publicat a:J Biomol NMR
Autors principals: Saxena, Saurabh, Stanek, Jan, Cevec, Mirko, Plavec, Janez, Koźmiński, Wiktor
Format: Artigo
Idioma:Inglês
Publicat: Springer Netherlands 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4642592/
https://ncbi.nlm.nih.gov/pubmed/26409925
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-015-9989-5
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