Mechanical fibrinogen-depletion supports heparin-free mesenchymal stem cell propagation in human platelet lysate

BACKGROUND: Pooled human platelet lysate (pHPL) is an efficient alternative to xenogenic supplements for ex vivo expansion of mesenchymal stem cells (MSCs) in clinical studies. Currently, porcine heparin is used in pHPL-supplemented medium to prevent clotting due to plasmatic coagulation factors. We...

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Detalhes bibliográficos
Publicado no:J Transl Med
Main Authors: Laner-Plamberger, Sandra, Lener, Thomas, Schmid, Doris, Streif, Doris A., Salzer, Tina, Öller, Michaela, Hauser-Kronberger, Cornelia, Fischer, Thorsten, Jacobs, Volker R., Schallmoser, Katharina, Gimona, Mario, Rohde, Eva
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2015
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4641400/
https://ncbi.nlm.nih.gov/pubmed/26554451
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12967-015-0717-4
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