Human Adult Retinal Pigment Epithelial Stem Cell–Derived RPE Monolayers Exhibit Key Physiological Characteristics of Native Tissue

PURPOSE: We tested what native features have been preserved with a new culture protocol for adult human RPE. METHODS: We cultured RPE from adult human eyes. Standard protocols for immunohistochemistry, electron microscopy, electrophysiology, fluid transport, and ELISA were used. RESULTS: Confluent m...

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Vydáno v:Invest Ophthalmol Vis Sci
Hlavní autoři: Blenkinsop, Timothy A., Saini, Janmeet S., Maminishkis, Arvydas, Bharti, Kapil, Wan, Qin, Banzon, Tina, Lotfi, Mostafa, Davis, Janine, Singh, Deepti, Rizzolo, Lawrence J., Miller, Sheldon, Temple, Sally, Stern, Jeffrey H.
Médium: Artigo
Jazyk:Inglês
Vydáno: The Association for Research in Vision and Ophthalmology 2015
Témata:
Retinal Cell Biology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4640474/
https://ncbi.nlm.nih.gov/pubmed/26540654
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1167/iovs.14-16246
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