Confluent Monolayers of Cultured Human Fetal Retinal Pigment Epithelium Exhibit Morphology and Physiology of Native Tissue

PURPOSE: Provide a reproducible method for culturing confluent monolayers of hfRPE cells that exhibit morphology, physiology, polarity, and protein expression patterns similar to native tissue. METHODS: Human fetal eyes were dissected on arrival, and RPE cell sheets were mechanically separated from...

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Hauptverfasser: Maminishkis, Arvydas, Chen, Shan, Jalickee, Stephen, Banzon, Tina, Shi, Guangpu, Wang, Fei E., Ehalt, Todd, Hammer, Jeffrey A., Miller, Sheldon S.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2006
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1904392/
https://ncbi.nlm.nih.gov/pubmed/16877436
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1167/iovs.05-1622
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