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Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography

Eight aminoacyl-tRNA synthetases (M, K, Q, D, R, I, EP and LARS) and three auxiliary proteins (AIMP1, 2 and 3) are known to form a multi-tRNA synthetase complex (MSC) in mammalian cells. We combined size exclusion chromatography (SEC) with reversed-phase liquid chromatography multiple reaction monit...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Park, Seong-Jun, Ahn, Hee-Sung, Kim, Jun Seok, Lee, Cheolju
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4636271/
https://ncbi.nlm.nih.gov/pubmed/26544075
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0142253
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