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A Simple Method for Quick-Freezing
In conventional freeze-fracture replicas produced from tissue cryoprotected with glycerol, the hydrophobic inner surfaces of membranes are revealed, but hydrophillic structures are obscured in the surrounding ice. Quick-freezing of tissue obviates the need for glycerol, which prevents the removal of...
Gespeichert in:
| Veröffentlicht in: | J Electron Microsc Tech |
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| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1986
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4636126/ https://ncbi.nlm.nih.gov/pubmed/26549928 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jemt.1060030210 |
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