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A Simple Method for Quick-Freezing

In conventional freeze-fracture replicas produced from tissue cryoprotected with glycerol, the hydrophobic inner surfaces of membranes are revealed, but hydrophillic structures are obscured in the surrounding ice. Quick-freezing of tissue obviates the need for glycerol, which prevents the removal of...

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Detalhes bibliográficos
Publicado no:J Electron Microsc Tech
Main Authors: BEARER, ELAINE L., ORCI, LELIO
Formato: Artigo
Idioma:Inglês
Publicado em: 1986
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4636126/
https://ncbi.nlm.nih.gov/pubmed/26549928
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jemt.1060030210
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