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Simultaneous determination of sample thickness, tilt, and electron mean free path using tomographic tilt images based on Beer-Lambert law

Cryo-electron tomography (cryo-ET) is an emerging technique that can elucidate the architecture of macromolecular complexes and cellular ultrastructure in a near-native state. Some important sample parameters, such as thickness and tilt, are needed for 3-D reconstruction. However, these parameters c...

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Detalhes bibliográficos
Publicado no:J Struct Biol
Main Authors: Yan, Rui, Edwards, Thomas J., Pankratz, Logan M., Kuhn, Richard J., Lanman, Jason K., Liu, Jun, Jiang, Wen
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4633387/
https://ncbi.nlm.nih.gov/pubmed/26433027
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2015.09.019
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