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Generation of a conditional analog-sensitive kinase in human cells using CRISPR/Cas9-mediated genome engineering

The ability to rapidly and specifically modify the genome of mammalian cells has been a long-term goal of biomedical researchers. Recently, the clustered, regularly interspaced, short palindromic repeats (CRISPR)/Cas9 system from bacteria has been exploited for genome engineering in human cells. The...

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Vydáno v:Methods Cell Biol
Hlavní autoři: Moyer, Tyler C., Holland, Andrew J.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2015
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4629829/
https://ncbi.nlm.nih.gov/pubmed/26175431
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mcb.2015.03.017
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