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T7 RNA polymerase mutants with altered promoter specificities.

The amino acid at position 748 in T7 RNA polymerase (RNAP) functions to discriminate base pairs at positions -10 and -11 in the promoter. We have constructed a series of T7 RNAP mutants having all possible amino acid substitutions at this position. Surprisingly, most (13/19) substitutions result in...

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Bibliographische Detailangaben
Hauptverfasser: Raskin, C A, Diaz, G A, McAllister, W T
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1993
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC46256/
https://ncbi.nlm.nih.gov/pubmed/8475053
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