T7 RNA polymerase mutants with altered promoter specificities.
The amino acid at position 748 in T7 RNA polymerase (RNAP) functions to discriminate base pairs at positions -10 and -11 in the promoter. We have constructed a series of T7 RNAP mutants having all possible amino acid substitutions at this position. Surprisingly, most (13/19) substitutions result in...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1993
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC46256/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8475053/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.8.3147 |
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