Soluble antigen profoundly reduces memory B-cell numbers even when given after challenge immunization.
The splenic B-cell repertoire of unimmunized C57BL/6 mice can be examined for anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) B cells of relatively high affinity by using a dual strategy. First, limiting numbers of splenocytes are polyclonally activated by Escherichia coli lipopolysaccharide and a mixture...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1993
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC46242/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8464928/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.7.3088 |
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