A comparison of DNA methylation specific droplet digital PCR (ddPCR) and real time qPCR with flow cytometry in characterizing human T cells in peripheral blood

Quantitating the copy number of demethylated CpG promoter sites of the CD3Z gene can be used to estimate the numbers and proportions of T cells in human blood and tissue. Quantitative methylation specific PCR (qPCR) is useful for studying T cells but requires extensive calibration and is imprecise a...

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Detalhes bibliográficos
Publicado no:Epigenetics
Main Authors: Wiencke, John K, Bracci, Paige M, Hsuang, George, Zheng, Shichun, Hansen, Helen, Wrensch, Margaret R, Rice, Terri, Eliot, Melissa, Kelsey, Karl T
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis 2014
Assuntos:
Research Paper
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4622657/
https://ncbi.nlm.nih.gov/pubmed/25437051
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/15592294.2014.967589
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