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Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9

Mouse spermatogonial stem cells (SSCs) can be cultured for multiplication and maintained for long periods while preserving their spermatogenic ability. Although the cultured SSCs, named germline stem (GS) cells, are targets of genome modification, this process remains technically difficult. In the p...

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Bibliografiske detaljer
Udgivet i:Stem Cell Reports
Main Authors: Sato, Takuya, Sakuma, Tetsushi, Yokonishi, Tetsuhiro, Katagiri, Kumiko, Kamimura, Satoshi, Ogonuki, Narumi, Ogura, Atsuo, Yamamoto, Takashi, Ogawa, Takehiko
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2015
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4618438/
https://ncbi.nlm.nih.gov/pubmed/26095606
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.stemcr.2015.05.011
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