Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9

Mouse spermatogonial stem cells (SSCs) can be cultured for multiplication and maintained for long periods while preserving their spermatogenic ability. Although the cultured SSCs, named germline stem (GS) cells, are targets of genome modification, this process remains technically difficult. In the p...

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Detalhes bibliográficos
Publicado no:Stem Cell Reports
Main Authors: Sato, Takuya, Sakuma, Tetsushi, Yokonishi, Tetsuhiro, Katagiri, Kumiko, Kamimura, Satoshi, Ogonuki, Narumi, Ogura, Atsuo, Yamamoto, Takashi, Ogawa, Takehiko
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2015
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4618438/
https://ncbi.nlm.nih.gov/pubmed/26095606
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.stemcr.2015.05.011
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