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Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity.

Bacteriophage T4 DNA polymerase has a proofreading 3'-->5' exonuclease that plays an important role in maintaining the accuracy of DNA replication. We have constructed a T4 DNA polymerase deficient in this exonuclease by converting Asp-219 to Ala. The exonuclease activity of the mutant...

Täydet tiedot

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Bibliografiset tiedot
Päätekijät: Frey, M W, Nossal, N G, Capson, T L, Benkovic, S J
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 1993
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC46138/
https://ncbi.nlm.nih.gov/pubmed/8464864
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