Isolation of Endogenously Assembled RNA-Protein Complexes Using Affinity Purification Based on Streptavidin Aptamer S1

Efficient isolation of endogenously assembled viral RNA-protein complexes is essential for understanding virus replication mechanisms. We have developed an affinity purification strategy based on an RNA affinity tag that allows large-scale preparation of native viral RNA-binding proteins (RBPs). The...

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Publié dans:Int J Mol Sci
Auteurs principaux: Dong, Yangchao, Yang, Jing, Ye, Wei, Wang, Yuan, Ye, Chuantao, Weng, Daihui, Gao, Huan, Zhang, Fanglin, Xu, Zhikai, Lei, Yingfeng
Format: Artigo
Langue:Inglês
Publié: MDPI 2015
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Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4613318/
https://ncbi.nlm.nih.gov/pubmed/26389898
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms160922456
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