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Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells

The in vitro RNA-pulldown is still largely used in the first steps of protocols aimed at identifying RNA-binding proteins that recognize specific RNA structures and motifs. In this RNA-pulldown protocol, commercially synthesized RNA probes are labeled with a modified form of biotin, desthiobiotin, a...

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書目詳細資料
發表在:J Vis Exp
Main Authors: Kresoja-Rakic, Jelena, Felley-Bosco, Emanuela
格式: Artigo
語言:Inglês
出版: MyJove Corporation 2018
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC6100806/
https://ncbi.nlm.nih.gov/pubmed/29757292
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57516
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