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Probing yeast polarity with acute, reversible, optogenetic inhibition of protein function

We recently developed a technique for rapidly and reversibly inhibiting protein function through light-inducible sequestration of proteins away from their normal sites of action. Here, we adapt this method for inducible inactivation of Bem1, a scaffold protein involved in budding yeast polarity. We...

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Publicat a:ACS Synth Biol
Autors principals: Jost, Anna Payne-Tobin, Weiner, Orion D.
Format: Artigo
Idioma:Inglês
Publicat: 2015
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4609243/
https://ncbi.nlm.nih.gov/pubmed/26035630
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.5b00053
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