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Probing yeast polarity with acute, reversible, optogenetic inhibition of protein function
We recently developed a technique for rapidly and reversibly inhibiting protein function through light-inducible sequestration of proteins away from their normal sites of action. Here, we adapt this method for inducible inactivation of Bem1, a scaffold protein involved in budding yeast polarity. We...
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| Udgivet i: | ACS Synth Biol |
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| Main Authors: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2015
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4609243/ https://ncbi.nlm.nih.gov/pubmed/26035630 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.5b00053 |
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