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Substoichiometric amounts of the molecular chaperones GroEL and GroES prevent thermal denaturation and aggregation of mammalian mitochondrial malate dehydrogenase in vitro.

The molecular chaperones GroEL and GroES were produced at very high levels in Escherichia coli, purified, and shown to protect pig mitochondrial malate dehydrogenase (MDH) against thermal inactivation in vitro. The apparent rate of MDH inactivation at 37 degrees C was reduced by a factor of at least...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Hartman, D J, Surin, B P, Dixon, N E, Hoogenraad, N J, Høj, P B
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1993
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC46069/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8096339/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.6.2276
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