Activity of purified NIFA, a transcriptional activator of nitrogen fixation genes.
The NIFA protein activates transcription of nitrogen fixation (nif) operons by the sigma 54-holoenzyme form of RNA polymerase. We purified active NIFA from Klebsiella pneumoniae in the form of a maltose-binding protein (MBP)-NIFA fusion; proteolytic release of MBP yielded inactive and insoluble NIFA...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1993
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC46067/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8460132/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.6.2266 |
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