Activity of purified NIFA, a transcriptional activator of nitrogen fixation genes.

The NIFA protein activates transcription of nitrogen fixation (nif) operons by the sigma 54-holoenzyme form of RNA polymerase. We purified active NIFA from Klebsiella pneumoniae in the form of a maltose-binding protein (MBP)-NIFA fusion; proteolytic release of MBP yielded inactive and insoluble NIFA...

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Bibliografische gegevens

Gepubliceerd in:	Proc Natl Acad Sci U S A
Hoofdauteurs:	Lee, H S, Berger, D K, Kustu, S
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	National Academy of Sciences 1993
Onderwerpen:	Research Article
Online toegang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC46067/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8460132/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.6.2266
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