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Convenient analysis of protein modification by chemical blotting with fluorogenic “click” reagents
Direct visualization of bioorthogonal alkyne or azide handles using fluorogenic azide-alkyne cycloaddition conducted on the surface of a blot membrane. The method eliminates the need for separation steps to remove excess small molecule reagents before attachment of antigen molecules or other visuali...
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| 出版年: | Mol Biosyst |
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| 主要な著者: | , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2015
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4605879/ https://ncbi.nlm.nih.gov/pubmed/26325302 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c5mb00510h |
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