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Determining the N-terminal orientations of recombinant transmembrane proteins in the Escherichia coli plasma membrane

In silico algorithms have been the common approach for transmembrane (TM) protein topology prediction. However, computational tools may produce questionable results and experimental validation has proven difficult. Although biochemical strategies are available to determine the C-terminal orientation...

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Detalles Bibliográficos
Publicado en:Sci Rep
Main Authors: Lee, Chien-Hsien, Chou, Chia-Cheng, Hsu, Min-Feng, Wang, Andrew H.-J.
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group 2015
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4604451/
https://ncbi.nlm.nih.gov/pubmed/26462555
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep15086
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