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Determining the N-terminal orientations of recombinant transmembrane proteins in the Escherichia coli plasma membrane

In silico algorithms have been the common approach for transmembrane (TM) protein topology prediction. However, computational tools may produce questionable results and experimental validation has proven difficult. Although biochemical strategies are available to determine the C-terminal orientation...

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Publicat a:Sci Rep
Autors principals: Lee, Chien-Hsien, Chou, Chia-Cheng, Hsu, Min-Feng, Wang, Andrew H.-J.
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group 2015
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4604451/
https://ncbi.nlm.nih.gov/pubmed/26462555
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep15086
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