Comparative evaluation of commercially available manual and automated nucleic acid extraction methods for rotavirus RNA detection in stools

Rotaviruses are a major cause of viral gastroenteritis in children. For accurate and sensitive detection of rotavirus RNA from stool samples by reverse transcription-polymerase chain reaction (RT-PCR), the extraction process must be robust. However, some extraction methods may not remove the strong...

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Bibliografische gegevens
Gepubliceerd in:J Virol Methods
Hoofdauteurs: Esona, Mathew D., McDonald, Sharla, Kamili, Shifaq, Kerin, Tara, Gautam, Rashi, Bowen, Michael D.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Elsevier/North-Holland Biomedical Press 2013
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Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4603280/
https://ncbi.nlm.nih.gov/pubmed/24036075
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2013.08.023
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