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Activity of select dehydrogenases with Sepharose-immobilized N(6)-carboxymethyl-NAD
N(6)-carboxymethyl-NAD (N(6)-CM-NAD) can be used to immobilize NAD onto a substrate containing terminal primary amines. We previously immobilized N(6)-CM-NAD onto sepharose beads and showed that Thermotoga maritima glycerol dehydrogenase could use the immobilized cofactor with cofactor recycling. We...
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| 發表在: | Bioengineered |
|---|---|
| Main Authors: | , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Taylor & Francis
2015
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4601513/ https://ncbi.nlm.nih.gov/pubmed/25611453 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/21655979.2014.1004020 |
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