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Activity of select dehydrogenases with Sepharose-immobilized N(6)-carboxymethyl-NAD

N(6)-carboxymethyl-NAD (N(6)-CM-NAD) can be used to immobilize NAD onto a substrate containing terminal primary amines. We previously immobilized N(6)-CM-NAD onto sepharose beads and showed that Thermotoga maritima glycerol dehydrogenase could use the immobilized cofactor with cofactor recycling. We...

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Detalhes bibliográficos
Publicado no:Bioengineered
Main Authors: Beauchamp, Justin, Vieille, Claire
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4601513/
https://ncbi.nlm.nih.gov/pubmed/25611453
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/21655979.2014.1004020
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